| Is hybridoma production about to take a quantum leap forward?
Two cultures derived from the same culture of a stable hybridoma clone were grown, one in NPD and the other in DI based medium supplemented with 3 percent FCS. Before seeding the cells were washed in serum-free media to verify the removal of any residual serum. During a period of two weeks the supernatants were collected as indicated and the cells were counted on the same day. The cultures were fed on the 4th and 10th day and medium was placed in the cultures on day 6. Although the cells in DI culture proliferated normally under these conditions, they failed to produce measurable quantities of antibody. Credit: Natalie Gavrilov-Yusim .
Teen isn't sure yet how experience as a monk has affected him
Teen isn't sure yet how experience as a monk has affected him By Christine Clarridge McClatchy Newspapers SEATTLE - Michael Sa-Ngoun is no longer a monk.The 19-year-old from Tukwila, Wash., who spent two years in a Cambodian monastery because his mother was desperate to stop his self-destructive behavior, is not prone to deep philosophical meditation these days.He doesn't work very hard to resist the desires of most young men his age, nor does he seek humility at every turn.It took surprisingly little time, family members say, for him to turn back into a regular American young man after his return from Cambodia a little more than a year ago. .
Slay That Green Dragon
Here on OpEdNEws, I haven't seen one single article supporting Hillary. It's not because we haven't accepted them. Matter of fact we welcome cogent, well written articles supporting ANY candidate, since we trust our readers to respond and balance out the perspective in the comments section. It appears that the concensus of the readers and writers on this site, most of whom were Kucinich, Edwards or Paul supporters, are, except for the Paul supporters, now leaning towards Obama. .
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